Counterimmunoelectrophoresis for the diagnosis of bovine mycoplasmosis [Mycoplasma bovis, Mycoplasma arginini]

Counterimmunoelectrophoresis (CIE) procedure has been established for mycoplasma identification from cultures, and the specificity and the sensitivity of this technique were evaluated. Preparations of milk samples and clinical and necropsy specimens were used for the technique. From 171 milk samples...

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Bibliographic Details
Published inAmerican journal of veterinary research Vol. 45; no. 5; p. 1020
Main Authors Bois, J.M, Roy, R.S, Guay, P
Format Journal Article
LanguageEnglish
Published United States 01.05.1984
Subjects
Animals
Antigens, Bacterial - analysis
Cattle
Counterimmunoelectrophoresis
Cross Reactions
Female
Immunoelectrophoresis
Mastitis, Bovine - diagnosis
Milk - microbiology
Mycoplasma - immunology
Mycoplasma Infections - diagnosis
Mycoplasma Infections - veterinary
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Summary:Counterimmunoelectrophoresis (CIE) procedure has been established for mycoplasma identification from cultures, and the specificity and the sensitivity of this technique were evaluated. Preparations of milk samples and clinical and necropsy specimens were used for the technique. From 171 milk samples, 80 (46.8%) were positive by CIE (73 samples with Mycoplasma bovis and 7 with Mycoplasma arginini) and 77 (45%) were positive by biochemical and growth inhibition tests (70 samples with Mycoplasma bovis and 7 with Mycoplasma arginini). From 36 mycoplasma culture-positive clinical and necropsy specimens, CIE identified 5 different mycoplasma species and from 3 mycoplasma culture-positive specimens, the technique identified coinfection by 2 mycoplasma species. The CIE is a reliable, specific, sensitive, and rapid technique for the diagnosis of mycoplasmosis in the bovine.
Bibliography:8522592
L73
ISSN:0002-9645
1943-5681