Development of Surface Plasmon Resonance Immunosensor through Metal Ion Affinity and Mixed Self-Assembled Monolayer

• Published in: Journal of microbiology and biotechnology Vol. 18; no. 10; pp. 1695 - 1700
• Main Authors: Lee, S.R. (Sogang University, Seoul, Republic of Korea), Sim, S.J. (Kwangwoon University, Seoul, Republic of Korea), Park, C.H. (Korea University, Seoul, Republic of Korea), Gu, M.B. (Dongyang University, Yeongju, Republic of Korea), Hwang, U.Y. (Seoul National University, Seoul, Republic of Korea), Yi, J.H. (Sogang University, Seoul, Republic of Korea), Oh, B.K. (Sogang University, Seoul, Republic of Korea), Lee, J.W. (Sogang University, Seoul, Republic of Korea), E-mail: jinwonlee@sogang.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.10.2008; 한국미생물·생명공학회
• Subjects: Antibodies - analysis; Biological and medical sciences; Biosensors; Biotechnology; Fundamental and applied biological sciences. Psychology; Gold - chemistry; human serum albumin; Humans; Immunoassay - methods; immunosensor; Ions - chemistry; metal ion; Methods. Procedures. Technologies; Mixed self-assembled monolayer; Palmitic Acids - chemistry; PROTEINAS; PROTEINE; PROTEINS; Serum Albumin - analysis; surface plasmon resonance; Surface Plasmon Resonance - methods; Surface Properties; Various methods and equipments; 생물학; Human; Self assembly; Monolayer; Metal ion; Serum albumin; Surface plasmon; Immunosensor; Mixed self-assembled monolayer; surface plasmon resonance; Biosensor; Affinity; Resonance; human serum albumin
• ISSN: 1017-7825

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16-mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO₃)₂ㆍ6H₂O immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate-buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy, conditions.