Amelioration of ozone-induced oxidative damage in wheat plants grown under high carbon dioxide. Role of antioxidant enzymes

• Published in: Plant physiology (Bethesda) Vol. 109; no. 2; pp. 421 - 432
• Main Authors: Rao, M.V. (University of Guelph, Guelph, Ontario, Canada.), Hale, B.A, Ormrod, D.P
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.10.1995
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AGENTES NOCIVOS; ANALISIS DE TEJIDOS; ANALYSE DE TISSUS; Animal, plant and microbial ecology; ANTIOXIDANTES; Antioxidants; ANTIOXYDANT; Applied ecology; Biological and medical sciences; BIOMASA; BIOMASSE; Carbon dioxide; CAROTENOIDE; CAROTENOIDES; CHLOROPHYLLE; CLOROFILAS; COENZIMAS; COENZYME; CRECIMIENTO; CROISSANCE; DIOXIDO DE CARBONO; DIOXYDE DE CARBONE; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on plants and fungi; Enzymes; FACTEUR NUISIBLE; FEUILLE; Fundamental and applied biological sciences. Psychology; Gels; HOJAS; ISOENZIMAS; ISOENZYME; Non agrochemicals pollutants; NUCLEOTIDE; NUCLEOTIDOS; OXIDACION; Oxidative stress; OXIDORREDUCTASAS; OXYDATION; OXYDOREDUCTASE; Oxygen; OZONE; OZONO; PEROXIDASAS; PEROXYDASE; Phytopathology. Animal pests. Plant and forest protection; Plant growth; Plants; Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors; Pollution effects. Side effects of agrochemicals; Protein isoforms; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; TRITICUM AESTIVUM; Whole Plant, Environmental, and Stress Physiology; Monocotyledones; Growth; Enzyme; Glutathione reductase (NADPH); Interaction; Carbon dioxide; Ozone; Superoxide dismutase; Biomass; Antioxidant; Cereal crop; Proteins; Peroxidases; Enzymatic activity; Gramineae; Angiospermae; Air pollution; Spermatophyta; Oxidation; Peroxidase; Oxidoreductases; Triticum aestivum
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.109.2.421

O3-induced changes in growth, oxidative damage to protein, and specific activities of certain antioxidant enzymes were investigated in wheat plant (Triticum aestivum L. cv Roblin) grown under ambient or high CO2. High CO2 enhanced shoot biomass of wheat plants, whereas O3 exposure decreased shoot biomass. The shoot biomass was relatively unaffected in plants grown under a combination of high CO2 and O3. O3 exposure under ambient CO2 decreased photosynthetic pigments, soluble proteins, and ribulose-1,5-bisphosphate carboxylase/oxygenase protein and enhanced oxidative damage to proteins, but these effects were not observed in plants exposed to O3 under high CO2. O3 exposure initially enhanced the specific activities of superoxide dismutase, peroxidase, glutathione reductase, and ascorbate peroxidase irrespective of growth in ambient or high CO2. However, the specific activities decreased in plants with prolonged exposure to O3 under ambient CO2 but not in plants exposed to O3 under high CO2. Native gels revealed preferential changes in the isoform composition of superoxide dismutase, peroxidases, and ascorbate peroxidase of plants grown under a combination of high CO2 and O3. Furthermore, growth under high CO2 and O3 led to the synthesis of one new isoform of glutathione reductase. This could explain why plants grown under a combination of high CO2 and O3 are capable of resisting O3-induced damage to growth and proteins compared to plants exposed to O3 under ambient CO2