Role of Tumour Necrosis Factor α in Stimulation of Prostaglandins F₂α and E₂ Release by Cultured Porcine Endometrial Cells

• Published in: Reproduction in domestic animals Vol. 41; no. 6; pp. 562 - 567
• Main Authors: Blitek, A, Ziecik, AJ
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2006; Blackwell Publishing Ltd; Blackwell Science
• Subjects: Biological and medical sciences; culture media; cultured cells; dosage; endometrium; epithelial cells; estrus; Fundamental and applied biological sciences. Psychology; hormone secretion; luteinizing hormone; luteolysis; Mammalian reproduction. General aspects; oxytocin; prostaglandins; swine; temporal variation; tumor necrosis factor-alpha; Vertebrates: reproduction; Arachidonic acid derivatives; Cytokine; Prostaglandin E2; Stimulation; In vitro; Pig; Prostaglandin F2α; Vertebrata; Mammalia; Uterus; Eicosanoid; Animal; Female genital system; Artiodactyla; Ungulata; Endometrium; Tumor necrosis factor α; Release
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2006.00716.x

The present studies were undertaken to examine the effect of tumour necrosis factor (TNF) α on prostaglandins (PGs) F₂α and E₂ release by cultured porcine endometrial cells harvested on days 13-16 after oestrus in comparison to stimulation with oxytocin (OT) and luteinizing hormone (LH). A time-dependent effect of TNFα (10 ng/ml) on PGF₂α release was observed in stromal and luminal epithelial cells. Moreover, TNFα increased PGF₂α secretion from both endometrial cell types with effective concentrations of 1 (p < 0.05), 10 and 50 ng/ml (p < 0.01). The effect of TNFα (10 ng/ml) on endometrial PGF₂α and PGE₂ release was compared with OT (100 nmol/l) and LH (100 ng/ml). All factors affected PGF₂α secretion from stromal cells, however, the stimulation tended to be more potent after OT and LH (p < 0.01) than after TNFα (p < 0.05) treatment. In epithelial cells, only TNFα was able to stimulate PGF₂α release (p < 0.001). PGE₂ secretion from stromal cells increased after incubation with TNFα and OT (p < 0.05). Only LH stimulated PGE₂ release from epithelium (p < 0.001), and its action was very effective when compared with TNFα or OT (p < 0.01). Summarizing, TNFα induces both PGs secretion from cultured porcine endometrium, but preferentially stimulates PGF₂α release from luminal epithelial cells. Therefore, similarly to OT and LH, TNFα may be considered as a potential modulator of endometrial PGF₂α production during luteolysis in the pig.