IGF-II and IGFBP-1 reversely regulate blastocyst implantation in mouse

• Published in: Chinese science bulletin Vol. 47; no. 21; pp. 1816 - 1820
• Main Authors: Tian, Yongqiang, Tie, Guodong, Cao, Yujing, Zhao, Xingxu, Duan, Enkui
• Format: Journal Article
• Language: English
• Published: Springer-Verlag 01.11.2002; State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China%State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China%College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China
• Subjects: coculture; embryo implantation; endometrium; epithelial cells; fluorescent antibody technique; insulin-like growth factor binding proteins; insulin-like growth factor II; mice; trophoblast; IGFBP-1; implantation; MMP-2; MMP-9; IGF-
• ISSN: 1001-6538; 1861-9541
• DOI: 10.1360/02tb9397

Insulin-like growth factor (IGF)-II and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF-II and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-II significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF-II enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of trophoblast and the inhibition of deciduas may be regulated by the interaction between the IGF-II-expressing invading cytotrophoblast and maternal deciduas-derived IGFBP-1.