Comparison of the suitability of derivatisation agents in HPLC - fluorescence detection analysis of fumonisins

• Published in: Journal of food and nutrition research Vol. 45; no. 3; pp. 127 - 133
• Main Authors: Dasko, L, Rauova, D, Belajova, E
• Format: Journal Article
• Language: English
• Published: 2006
• Subjects: BEERS; BIERE; CERVEZAS; ESPECTROMETRIA; FUMONISINAS; FUMONISINE; FUMONISINS; HPLC; http://www.fao.org/aos/agrovoc#c_24469; http://www.fao.org/aos/agrovoc#c_33096; http://www.fao.org/aos/agrovoc#c_36148; http://www.fao.org/aos/agrovoc#c_7283; http://www.fao.org/aos/agrovoc#c_864; REPUBLICA ESLOVACA; REPUBLIQUE SLOVAQUE; SLOVAK REPUBLIC; SPECTROMETRIE; SPECTROMETRY
• ISSN: 1336-8672

Suitability of derivatisation agents - o-phthaldialdehyde (OPA), 2-mercaptoethanol (MET), N-acetyl-L-cysteine (N-AC), 4-chloro-7-nitrobenzofurazan (NBD-Cl), and dansylchloride (DC) - for determination of fumonisins in model solutions and in beers has been studied measuring UV/VIS stability of reaction complexes as well as fluorescence characteristics. While DC adducts of fumonisins showed sufficient stability, OPA and MET gave the best fluorescence responses. However, stability of their reaction complex was much lower than that of the dansylated adduct. The mixture of OPA and N-AC provided comparable results to OPA and MET mixtures. Under given reaction conditions, the lowest fluorescence response of derivatised fumonisins was observed with NBD-Cl. Calibration lines for both fumonisins in standard solution were linear within the range 2.2--3000 micro g.l sup(-1) with the coefficient of linearity 0.9998.