Structural studies on the anti tumor active polysaccharides from Coriolus versicolor (Basidiomycetes). i. fractionation with barium hydroxide

• Published in: YAKUGAKU ZASSHI Vol. 96; no. 4; pp. 413 - 418
• Main Authors: Hirase, S, Nakai, S, Akatsu, T, Kobayashi, A, Oohara, M, Matsunaga, K.I, Fujii, M, Kodaira, S, Fujii, T
• Format: Journal Article
• Language: Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.04.1976
• Subjects: Animals; Antineoplastic Agents, Phytogenic - isolation & purification; Barium; Basidiomycota; Basidiomycota - analysis; Chemical Fractionation; Chemical Phenomena; Chemistry; Female; fungi; Hydroxides; Mice; Mice, Inbred ICR; Polysaccharides - isolation & purification
• ISSN: 0031-6903; 1347-5231
• DOI: 10.1248/yakushi1947.96.4_413

A polysaccharide mixture was extracted from the mycelium of Coriolus versicolor with hot water and precipitated with ethanol. Fractionation with increasing concentrations of barium hydroxide solution and then with a cetyltrimethylammonium hydroxide solution afforded five fractions, among which a fraction precipitating with 0.3N barium hydroxide solution was found to have a marked antitumor activity against sarcoma-180 implanted in mice. This fraction was found to be composed of β-D-glucan and a smaller amount of protein, which could not be separated from the glucan moiety by any of the Sevag method, precipitation with trifluorotrichloroethane, digestion with pronase, or chromatography on a column packed with molecular sieves. On hydrolysis, this fraction gave D-glucose as the only carbohydrate component together with common amino acids. A β-glycosidic linkage was suggested from a low value of its specific optical rotation.