Similarities in restriction fragment patterns of mitochondrial DNAs of Phytophthora species strongly depend on the restriction enzyme used due to heterogeneous base distribution and sequence conservation

• Published in: Fungal genetics and biology Vol. 20; no. 1; pp. 36 - 42
• Main Authors: Cock, A.W.A.M. de, Karlovsky, P, Jahnke, K.D, Bahnweg, G
• Format: Journal Article
• Language: English
• Published: 01.03.1996
• Subjects: chemotaxonomy; mitochondrial DNA; Phytophthora cactorum; Phytophthora capsici; Phytophthora cryptogea; Phytophthora drechsleri; Phytophthora infestans; restriction fragment length polymorphism; restriction mapping
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1006/fgbi.1996.0007

Mitochondrial DNAs of six morphologically different Phytophthora species were digested with 15 restriction enzymes. The numbers of restriction fragments obtained differed considerably from those theoretically expected for random base distribution. Enzymes with relatively many G and C in their recognition sequences produced significantly larger numbers of fragments. Moreover, fragments generated by most of these enzymes were more often shared by two or more species than those from enzymes with more A and T in their recognition sequence. It is concluded that base distribution in mitochondrial DNA of Phytophthora is heterogeneous, AT-rich stretches occurring scattered over the mitochondrial genome and GC-rich regions present in conserved sequences, presumably genes. A practical consequence for taxonomic RFLP studies is that optimal enzymes can be selected, depending on the desired level of resolution.