In Vitro Immunopotentiating Activities of Cellular Fractions of Lactic Acid Bacteria Isolated from Kimchi and Bifidobacteria

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 5; pp. 661 - 666
• Main Authors: Hur, H.J. (Seoul National University, Seoul, Republic of Korea), Lee, K.W. (Seoul National University, Seoul, Republic of Korea), Kim, H.Y. (Kyung Hee University, Suwon, Republic of Korea), Chung, D.K. (Kyung Hee University, Suwon, Republic of Korea), Lee, H.J. (Seoul National University, Seoul, Republic of Korea), E-mail: leehyjo@snu.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.05.2006
• Subjects: bifidobacteria; Biological and medical sciences; Biotechnology; CELL WALLS; CITOPLASMA; CYTOPLASM; CYTOPLASME; Fundamental and applied biological sciences. Psychology; heat-killed whole cells; immunopotentiating activities; Kimchi lactic acid bacteria; PARED CELULAR; PAROI CELLULAIRE; Lactic acid bacteria; Kimchi lactic acid bacteria; bifidobacteria; Bifidobacterium; Actinomycetaceae; Biological activity; Cell wall; heat-killed whole cells; Actinomycetales; Heat; Bacteria; Actinomycetes; immunopotentiating activities; Cytoplasm
• ISSN: 1017-7825

The present study represents the investigation of in vitro immunopotentiating activities of cellular fractions of major lactic acid bacteria found in kimchi (KLAB) and bifidobacteria. The macrophage cells, RAW264.7, were stimulated with heat-killed whole-cell, cell-wall, and cytoplasmic fractions of four strains of KLAB (Leuconostoc mesenteroides, Leuconostoc citreum, Lactobacillus plantarum, and Lactobacillus sake) and two strains of bifidobacteria (Bifidobacterium longum and Bifidobacterium lactis) each, and then the production of nitric oxide (NO) and cytokines including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was measured by Griess and ELISA assays, respectively.