Cloning and Characterization of Cyclohexanol Dehydrogenase Gene from Rhodococcus sp. TK6
The cyclohexanol dehydrogenase (ChnA), produced by Rhodococcus sp. TK6, which is capable of growth on cyclohexanol as the sole carbon source, has been previously purified and characterized. However, the current study cloned the complete gene (chnA) for ChnA and its flanking regions using a combinati...
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Published in | Journal of microbiology and biotechnology Vol. 15; no. 6; pp. 1189 - 1196 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Seoul
Korean Society for Applied Microbiology
01.12.2005
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Subjects | |
Online Access | Get full text |
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Summary: | The cyclohexanol dehydrogenase (ChnA), produced by Rhodococcus sp. TK6, which is capable of growth on cyclohexanol as the sole carbon source, has been previously purified and characterized. However, the current study cloned the complete gene (chnA) for ChnA and its flanking regions using a combination of a polymerase chain reaction (PCR) based oil the N-terminal amino acid sequence of the purified ChnA and plaque hybridization from a phage library of Rhodococcus sp. TK6. |
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Bibliography: | 2006015323 Q60 |
ISSN: | 1017-7825 |