Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro

• Published in: Journal of animal science Vol. 82; no. 6; pp. 1847 - 1854
• Main Authors: Lila, Z.A, Mohammed, N, Yasui, T, Kurokawa, Y, Kanda, S, Itabashi, H
• Format: Journal Article
• Language: English
• Published: Savoy, IL Am Soc Animal Sci 01.06.2004; American Society of Animal Science
• Subjects: Ammonia - metabolism; Animal Feed; Animal Nutritional Physiological Phenomena; Animal productions; Animals; Bacteria, Anaerobic - metabolism; Biological and medical sciences; Cattle; Colony Count, Microbial; Eukaryota - growth & development; Fatty Acids, Volatile - analysis; Fatty Acids, Volatile - biosynthesis; Female; Fermentation; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; In Vitro Techniques; Probiotics; Rumen - chemistry; Rumen - metabolism; Rumen - microbiology; Rumen - parasitology; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - physiology; Starch - metabolism; Terrestrial animal productions; Vertebrates; Methane; Probiotic; Ruminal Fermentation; Protozoa; Yeast; Fermentation; In vitro; Twin Strain of Saccharomyces cerevisiae; Fungi; Probiotics; Ascomycetes; Microorganism; Cell; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/2004.8261847x

This experiment was designed to investigate the effects of different concentrations (0, 0.33, 0.66, 0.99, and 1.32 g/L) of a twin-strain of Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation of corn starch, soluble potato starch, and sudangrass hay (60.5%, DM basis) plus concentrate mixture (39.5%, DM basis). Ruminal fluid was collected from two dairy cows, mixed with phosphate buffer (1:2), and incubated (30 mL) anaerobically at 38 degrees C for 6 and 24 h with or without yeast supplement, using 200 mg (DM basis) of each substrate. Medium pH, ammonia-N, and numbers of protozoa were unaffected (P = 0.38) by yeast cells in all substrates. Molar proportion of acetate was unchanged (P = 0.56) with cornstarch and soluble potato starch, but increased quadratically (P = 0.02) with hay plus concentrate by treatment. Addition of yeast cells caused a linear increase of total VFA (P = 0.008) in all substrates. Excluding the soluble potato starch, supplementation of S. cerevisiae resulted in a quadratic increase of propionate (P = 0.01), with a quadratic decrease (P = 0.04) of acetate:propionate. When soluble potato starch was used as a substrate, a linear increase (P = 0.006) of the molar proportion of propionate and a quadratic decrease (P = 0.007) in acetate:propionate was observed by treatment. Molar proportion of butyrate was unchanged (P = 0.35) with cornstarch and soluble potato starch, whereas it decreased linearly (P = 0.007) with hay plus concentrate by yeast cell supplementation. When cornstarch and soluble potato starch were used as a substrate, minor VFA were decreased (P = 0.05) by treatment. Accumulation of lactate was linearly decreased by treatment (P = 0.007) in all substrates. During incubation with hay plus concentrate, IVDMD was linearly increased (P = 0.006), whereas production of methane (linear; P = 0.02) and accumulation of hydrogen was decreased (quadratic; P = 0.005) by treatment after 24 h. These results showed that a twin strain of S. cerevisiae live cells stimulated in vitro mixed ruminal microorganism fermentation with decreased lactate, and a small decrease of methane and hydrogen with hay plus concentrate.