Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis

To find potential targets of novel antimicrobial agents, we identified essential genes of Staphylococcus aureus N315 by using comparative genomics and allele replacement mutagenesis. By comparing the genome of S. aureus N315 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, P...

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Published inJournal of microbiology and biotechnology Vol. 16; no. 4; pp. 623 - 632
Main Authors Ko, K.S. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), Lee, J.Y. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), Song, J.H. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), E-mail: jhsong@smc.samsung.co.kr, Baek, J.Y. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), Oh, W.S. (Sungkyunkwan University School of Medicine, Seoul, Republic of Korea), Chun, J.S. (Seoul National University, Seoul, Republic of Korea), Yoon, H.S. (LG Life Sciences, Inc., Daejeon, Republic of Korea)
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Applied Microbiology 01.04.2006
Subjects
allelic replacement mutagenesis
Biological and medical sciences
Biotechnology
essential genes
Fundamental and applied biological sciences. Psychology
genomics
new antimicrobial agent
STAPHYLOCOCCUS AUREUS
Genomics
allelic replacement mutagenesis
essential genes
Antimicrobial agent
Screening
new antimicrobial agent
Gene
Mutagenesis
Bacteria
Micrococcales
Micrococcaceae
Comparative study
Staphylococcus aureus
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Summary:To find potential targets of novel antimicrobial agents, we identified essential genes of Staphylococcus aureus N315 by using comparative genomics and allele replacement mutagenesis. By comparing the genome of S. aureus N315 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pneumoniae, a total of 481 candidate target genes with similar amino acid sequences with at least three other species by greater than 40% sequence identity were selected. Of 481 disrupted candidate genes, 122 genes were identified as essential genes for growth of S. aureus N315.
Bibliography:2007002654
A50
ISSN:1017-7825