Identification of Potential Corynebacterium ammoniagenes Purine Gene Regulators Using the pur-lacZ Reporter in Escherichia coli

• Published in: Journal of microbiology and biotechnology Vol. 14; no. 6; pp. 1249 - 1255
• Main Authors: Han, R.N. (Yonsei University, Seoul, Republic of Korea), Chung, S.O. (CJ Co., Ichon, Republic of Korea), Kim, S.K. (Konkuk University, Seoul, Republic of Korea), Cho, I.H. (Yonsei University, Seoul, Republic of Korea), Choi, K.Y. (Yonsei University, Seoul, Republic of Korea), E-mail: kychoi@yonsei.ac.kr, Han, J.K. (CJ Co., Ichon, Republic of Korea), Lee, J.H. (CJ Co., Ichon, Republic of Korea)
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.12.2004
• Subjects: Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Biotechnology; Corynebacterium ammoniagenes; Fundamental and applied biological sciences. Psychology; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; purE; purF; PURINAS; PURINE; PURINES; reporter; reporter system; repressor; repressor; Escherichia coli; Regulator gene; Biosynthesis; Identification; reporter; de novo purine nucleotide biosynthesis; purE; purF; Nucleotide; Corynebacterium ammoniagenes; Bacteria; reporter system; Purine; Enterobacteriaceae
• ISSN: 1017-7825

This study has developed Corynebacterium ammoniagenes (c. ammoniagenes) purine gene transcriptional reporters (purF-lacZ and purE-lacZ) that function in Escherichia coli (E. coli) DH5a. After transformation of a C. ammoniagenes gDNA library into E. coli cells harboring either purF-lacZ or purE-lacZ, C. ammoniagenes clones were obtained that repress purF-lacZ and purE-lacZ gene expression. The potential purE and purF regulatory genes are homologous to the genes encoding transcription regulators, the regulatory subunit of RNA polymerase, and genes for purine nucleotide biosynthesis of various bacteria.