Establishment of callus and cell suspension cultures of Opuntia ficus-indica

Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 micromolar 6-furfurylaminopu...

Full description

Saved in:
Bibliographic Details
Published inPlant cell, tissue and organ culture Vol. 58; no. 2; pp. 155 - 157
Main Authors Llamoca-Zarate, R.M, Studart-Guimaraes, C, Landsmann, J, Campos, F.A.P
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 1999
Subjects
2,4-D
aromatic acids
Biological and medical sciences
Biotechnology
callus
casein hydrolysate
cell suspension culture
cotyledons
culture media
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
explants
Fundamental and applied biological sciences. Psychology
hypocotyls
Methods. Procedures. Technologies
micropropagation
Opuntia ficus-indica
Plant cells and fungal cells
purines
sucrose
tissue culture
Cell culture
Callus
Vegetals
Cactaceae
Fruit crop
Dicotyledones
Angiospermae
Spermatophyta
Opuntia ficus indica
Cell line establishment
Online AccessGet full text

Cover

More Information
Summary:Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 micromolar 6-furfurylaminopurine, 2.3 micromolar 2,4-dichlorophenoxyacetic acid, 1.0 micromolar 4-amino 3,5,6-trichloropicolinic acid, 400 mg/l casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.
ISSN:0167-6857
1573-5044
DOI:10.1023/A:1006315729266