Selection of downy mildew resistant somaclones, from a susceptible B line of pearl millet

• Published in: Plant cell, tissue and organ culture Vol. 58; no. 2; pp. 159 - 162
• Main Authors: Umesha, S, Nagarathna, K.C, Shetty, S.A, Shetty, H.S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1999
• Subjects: agronomic traits; Biological and medical sciences; biological resistance; Biotechnology; clones; disease resistance; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Pennisetum glaucum; Plant cells and fungal cells; regrowth; Sclerospora graminicola; somaclonal variation; tissue culture; Agronomic character; Monocotyledones; Vegetals; Plant pathogen; Phycomycetes; Pennisetum glaucum; Sclerospora graminicola; Tissue culture; Somaclonal variation; Cereal crop; Fungus resistance; Fungi; Regeneration; Gramineae; Angiospermae; Genetic improvement; Spermatophyta; Thallophyta
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1023/A:1006347113434

Plants regenerated from seed-derived callus of a PNMS 6B line of pearl millet (Pennisetum glaucum (L.) R. Br.) were evaluated for their resistance induced by somaclonal variation for downy mildew disease caused by Sclerospora graminicola (Sacc.) Schroter. Among the 201 lines regenerated, only 3 lines consistently proved highly resistant (free from disease incidence) for up to 5 generations; whereas, 17 lines were resistant (disease by incidence ranging from 1 to 9%). Resistance was confirmed by testing the plants under both laboratory and field conditions. The plants were evaluated for their agronomic traits.