Cloning of a Novel Na+-Dependent L-Serine Specific Symporter Gene from Haemophilus influenzae Rd and Characteristics of the Transporter

A protein that exhibited a high similarity to a major serine transporter of Escherichia coli, SdaC, was found in Haemophilus injluenzae Rd. Also, Na+-stimulated serine transport activity was detected in the cells. The sdaC of H. injluenzae was cloned and the properties of the transporter were invest...

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Published inJournal of microbiology and biotechnology Vol. 14; no. 3; pp. 520 - 524
Main Authors Kim, Y.M. (Kyungpook National University, Daegu, Republic of Korea), E-mail: ymkimchoi@bh.knu.ac.kr, Tsuchiya, Tomofusa (Okayama University, Okayama, Japan), Rhee, I.K. (Kyungpook National University, Daegu, Republic of Korea)
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Applied Microbiology 01.06.2004
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
HAEMOPHILUS
Haemophilus influenzae Rd
Na+-dependent L-serine specific symporter
SERINA
SERINE
Pasteurellaceae
Gene
Na+-dependent L- serine specific symporter
Serine
Bacteria
Haemophilus influenzae Rd
Haemophilus influenzae
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Summary:A protein that exhibited a high similarity to a major serine transporter of Escherichia coli, SdaC, was found in Haemophilus injluenzae Rd. Also, Na+-stimulated serine transport activity was detected in the cells. The sdaC of H. injluenzae was cloned and the properties of the transporter were investigated. The activity of serine transport was stimulated by Na+. Uptake of Na+ elicited by L-serine influx into cells was also observed, which supports the idea that L-serine is transported by a mechanism of Na+ /serine symport.
Bibliography:2005012051
T10
ISSN:1017-7825