Investigation of Possible Gene Transfer to Soil Microorganisms for Environmental Risk Assessment of Genetically Modified Organisms

• Published in: Journal of microbiology and biotechnology Vol. 14; no. 3; pp. 498 - 502
• Main Authors: Kim, Y.T. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Park, B.K. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Hwang, E.I. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Yim, N.H. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Kim, N.R. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Kang, T.H. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Lee, S.H. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), Kim, S.U. (Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea), E-mail: kimsu@kribb.re.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.06.2004
• Subjects: antibiotic-resistant gene; Biological and medical sciences; Biotechnology; ENVIRONMENTAL IMPACT ASSESSMENT; EVALUACION DEL IMPACTO AMBIENTAL; EVALUATION IMPACT SUR ENVIRONNEMENT; Fundamental and applied biological sciences. Psychology; GENE TRANSFER; Genetically modified organisms; small-scale soil-core device; soil bacteria; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Soils; gene transfer; Genetically modified organisms; Microorganism; Risk analysis; Genetically modified organism; antibiotic-resistant gene; environmental risk assessment; small-scale soil-core device; soil bacteria; Genetic transfer
• ISSN: 1017-7825

The current study was conducted to monitor the possibility of the gene transfer among soil bacteria, including the effect of drift due to rain and surface water, in relation to the release of genetically modified organisms into the environment. Four types of bacteria, each with a distinct antibiotic marker, kanamycin-resistant P. fluorescens, rifampicin-resistant P. putida, chloramphenicol-resistant B. subtilis, and spectinomycin-resistant B. subtilis, were plated using a small-scale soil-core device designed to track drifting microorganisms.