Cloning and analysis of piRNAs from testes of three species

piRNAs are a testis-specific class of small RNAs in mammal. Sixteen sequences of piRNAs were cloned from three different species by the method of construction of cDNA library including two sequences from mouse, eight sequences from quail and six sequences from chicken. Herein, the two piRNAs sequenc...

Full description

Saved in:
Bibliographic Details
Published inWētchasān sattawaphāet Vol. 41; no. 2; p. 193
Main Authors Chang, Guobin, Chen, Rong, Zhang, Ying, Dai, Aiqin, Ma, Teng, Chen, Guohong
Format Journal Article
LanguageEnglish
Published Bangkok Chulalongkorn University, Faculty of Veterinary Science 01.06.2011
Subjects
Cell cycle
Chicken
Chickens
Cloning
Mice
Nucleotide sequence
piRNAs
Poultry
Quail
Quails
RNA
Sperm
Testes
Testis
Online AccessGet full text

Cover

More Information
Summary:piRNAs are a testis-specific class of small RNAs in mammal. Sixteen sequences of piRNAs were cloned from three different species by the method of construction of cDNA library including two sequences from mouse, eight sequences from quail and six sequences from chicken. Herein, the two piRNAs sequences from mouse had already been identified, while the others were cloned for the first time. The sequence analysis indicated that the avian piRNAs had a similar length and a preference of uridine for 5'-end with varying degrees to the mammalian piRNAs. Beside the function in the process of sperm development and maturation, avian piRNAs may also have a role in regulating the development of nerve and tumor cells. [PUBLICATION ABSTRACT]
Bibliography:L10
http://www.vet.chula.ac.th/~tjvm/browse.htm
ISSN:0125-6491