Flooding tolerance of transgenic tomato plants expressing the bacterial enzyme ACC deaminase controlled by the 35S, rolD or PRB-1b promoter

• Published in: Plant physiology and biochemistry Vol. 39; no. 1; pp. 19 - 25
• Main Authors: Grichko, V.P. (University of Waterloo (Canada). Department of Biology), Glick, B.R
• Format: Journal Article
• Language: English
• Published: Paris Elsevier 01.01.2001
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; ANOXIA; ANOXIE; Biological and medical sciences; DEAMINATION; DESAMINACION; DESAMINATION; ENZIMAS; ENZYME; ENZYMES; ESTRES; ETHYLENE; ETILENO; FLOODING; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetics and breeding of economic plants; HORTALIZAS (PLANTAS); INONDATION; INUNDACION; LYCOPERSICON ESCULENTUM; PLANTAS TRANSGENICAS; PLANTE LEGUMIERE; PLANTE TRANSGENIQUE; RESISTANCE AUX FACTEURS NUISIBLES; RESISTANCE TO INJURIOUS FACTORS; RESISTENCIA A AGENTES DANINOS; STRESS; TRANSGENIC PLANTS; Varietal selection. Specialized plant breeding, plant breeding aims; VEGETABLE CROPS; 1-Aminocyclopropanecarboxylic acid; Growth; Ethylene; Environmental factor; Lyases; Dicotyledones; Angiospermae; Lycopersicon esculentum; Plant growth substance; Transgenic plant; Solanaceae; 1-Aminocyclopropane-1-carboxylate deaminase; Adaptation; Root; Enzyme; Tolerance; Flooding; Survival; Stress; Carbon-carbon lyases; Sensitivity resistance; Vegetable crop; Metabolic precursor; Genetic improvement; Hypoxia; Spermatophyta
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/S0981-9428(00)01217-1

Transgenic tomato plants Lycopersicon esculentum (Solanaceae) cv. Heinz 902 expressing the bacterial gene 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, under the transcriptional control of either two tandem 35S cauliflower mosaic virus promoters (constitutive expression), the rolD promoter from Agrobacterium rhizogenes (root specific expression) or the pathogenesis related PRB-lb promoter from tobacco, were compared to non-transformed plants in their response to flooding stress. Characteristics that were studied include shoot height, and fresh and dry weight; epinasty; ACC deaminase activity; ethylene production; and leaf chlorophyll and protein content. All of the transgenic tomato plants expressing ACC deaminase showed some increased tolerance to flooding stress and were less subject to the deleterious effects of root hypoxia on plant growth than were non-transformed plants. Plants that included an ACC deaminase gene under the control of the rolD promoter were protected to the greatest extent