Evaluation of an HPLC method for the determination of phylloquinone (vitamin K1) in various food matrices

• Published in: Journal of agricultural and food chemistry Vol. 42; no. 2; pp. 295 - 300
• Main Authors: Booth, S.L, Davidson, K.W, Sadowski, J.A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.02.1994
• Subjects: ALIMENTOS; Biological and medical sciences; CARNE DE RES; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS; CROMATOGRAFIA LIQUIDA ALTA PRESION; EPINARD; ESPINACA; FLUORESCENCE; FLUORESCENCIA; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; JUGO DE LEGUMBRES; JUS DE LEGUME; LAIT; LECHE; METHODE D'ESSAI; Methods of analysis, processing and quality control, regulation, standards; METODOS DE ENSAYO; PAIN; PAN; PRODUIT ALIMENTAIRE; PURIFICACION; PURIFICATION; VIANDE BOVINE; VITAMINA K; VITAMINE K; Reduction; Analysis method; Vitamin; HPLC chromatography; Phylloquinone; Foodstuff; Application; Fluorescence spectrometry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00038a013

The amount of phylloquinone (vitamin K1) in five different foods was determined by applying a highly sensitive HPLC method that incorporates postcolumn chemical reduction of the quinone followed by fluorescence detection of the hydroquinone form of the vitamin. After initial extraction in 2-propanol and hexane, the food extracts were purified by solid-phase extraction (SPE) on silica gel and analyzed by HPLC or, depending on the matrix, further purified using liquid-phase reductive extraction or reversed-phase SPE on C18. Intra- and interday precision of the assay ranged from 6.6 to 13.6%. The between sample coefficient of variation in the phylloquinone content of 10 random bulk samples ranged from 7.8 to 44.6%, depending on the food item analyzed. In addition, endogenous dihydrophylloquinone was identified in foods containing hydrogenated oils, although its biological activity is unknown