Production and properties of L-asparaginase from Mucor species associated with a marine sponge (Spirastrella sp.)
The fungus Mucor sp. isolated from the marine sponge Spirastrella sp. produced extracellular L-asparaginase. The maximum L-asparaginase activity was found at 50 degrees C. The activation and deactivation energies of the partially purified enzyme were 9.58 and 21.69 kcal mol-1, respectively. The enzy...
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Published in | Cytobios Vol. 92; no. 370/371; pp. 165 - 173 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
1997
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Subjects | |
Online Access | Get more information |
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Summary: | The fungus Mucor sp. isolated from the marine sponge Spirastrella sp. produced extracellular L-asparaginase. The maximum L-asparaginase activity was found at 50 degrees C. The activation and deactivation energies of the partially purified enzyme were 9.58 and 21.69 kcal mol-1, respectively. The enzyme activity was not affected by the addition of 4% (w/v) NaCl and different metal ions (Co+2, Fe+2, Mg+2, Ni+2, and Zn+2) at 25 mmol l-1 but was strongly inhibited by EDTA. |
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ISSN: | 0011-4529 |