Production and properties of L-asparaginase from Mucor species associated with a marine sponge (Spirastrella sp.)

The fungus Mucor sp. isolated from the marine sponge Spirastrella sp. produced extracellular L-asparaginase. The maximum L-asparaginase activity was found at 50 degrees C. The activation and deactivation energies of the partially purified enzyme were 9.58 and 21.69 kcal mol-1, respectively. The enzy...

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Bibliographic Details
Published inCytobios Vol. 92; no. 370/371; pp. 165 - 173
Main Authors Mohapatra, B.R, Bapuji, M, Banerjee, U.C
Format Journal Article
LanguageEnglish
Published England 1997
Subjects
Animals
aquatic organisms
Asparaginase - chemistry
Asparaginase - isolation & purification
Carbon
Cations, Divalent
Chelating Agents - pharmacology
Edetic Acid - pharmacology
Enzyme Stability
Hydrogen-Ion Concentration
Mucor - enzymology
Nitrogen
plant biochemistry
plant physiology
Porifera - microbiology
Sodium Chloride - pharmacology
Temperature
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Summary:The fungus Mucor sp. isolated from the marine sponge Spirastrella sp. produced extracellular L-asparaginase. The maximum L-asparaginase activity was found at 50 degrees C. The activation and deactivation energies of the partially purified enzyme were 9.58 and 21.69 kcal mol-1, respectively. The enzyme activity was not affected by the addition of 4% (w/v) NaCl and different metal ions (Co+2, Fe+2, Mg+2, Ni+2, and Zn+2) at 25 mmol l-1 but was strongly inhibited by EDTA.
ISSN:0011-4529