Effect of emulsion formulation of sinorhizobium meliloti and pre-inoculated seeds on alfalfa nodulation and growth: a pouch study

• Published in: Journal of plant nutrition Vol. 36; no. 2; pp. 231 - 242
• Main Authors: John, Rojan P, Tyagi, R. D, Brar, Satinder K, Prévost, D, Surampalli, R. Y
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis Group 01.01.2013; Taylor & Francis Ltd
• Subjects: alfalfa; alfalfa growth; nodulation; nodulation index; plant growth; pouches; pre-inoculated seeds; seeds; Sinorhizobium meliloti; Sinorhizobium meliloti emulsion
• ISSN: 1532-4087; 0190-4167; 1532-4087
• DOI: 10.1080/01904167.2012.739243

Emulsion formulation found to be optimal for survival of Sinorhizobium meliloti and the efficiency was tested for the nodulation on alfalfa. The emulsion formulation can be diluted up to 10⁵ to 10⁷ times to achieve maximum nodulation index (18) and to get maximum growth of the plant (height and dry weight). Emulsion can be applied on seed to make pre-inoculated seeds and storage at 4°C for two months provides more than 10⁵ CFU/seeds (minimum standard requirement is 10³ CFU/seeds). Nodulation test proved that both minimum and maximum requirement provide highest nodulation index and almost similar growth effect after 28 days of growth in pouches.