Effect of ADP-ribosylation reaction on bull testis chromatin

Polynucleosomes prepared from bull testis nuclei were characterized: DNA length, by agarose gel electrophoresis, and distribution of poly(ADP-ribose)polymerase activity, by incubation with 0.64mM NAD were determined. Maximal activity was found in nucleosome fractions of 3-5 units. Chromatin fragment...

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Bibliographic Details
Published inBiochemistry and molecular biology international Vol. 31; no. 2; pp. 373 - 380
Main Authors Faraone-Mennella, M.R, De Lucia, F, Palomba, L, Quesada, P, Farina, B
Format Journal Article
LanguageEnglish
Published England 01.10.1993
Subjects
adenosine diphosphate
Adenosine Diphosphate Ribose - metabolism
Animals
bulls
Cattle
Cell Nucleus - ultrastructure
Centrifugation, Density Gradient
chromatin
Chromatin - chemistry
Chromatin - metabolism
Circular Dichroism
DNA - metabolism
Male
Nucleosomes - metabolism
Poly(ADP-ribose) Polymerases - metabolism
post-transcription modification
Protein Conformation
spectral analysis
testes
Testis - ultrastructure
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Summary:Polynucleosomes prepared from bull testis nuclei were characterized: DNA length, by agarose gel electrophoresis, and distribution of poly(ADP-ribose)polymerase activity, by incubation with 0.64mM NAD were determined. Maximal activity was found in nucleosome fractions of 3-5 units. Chromatin fragments (5-2 kbp polynucleosomes) were analysed by circular dichroism in both native and ADP-ribosylated forms. The spectrum of the endogenously ADP-ribosylated polynucleosomes, compared to the native fraction, exhibited a higher ellipticity value and a shift of the cross-over point towards the lowest wavelengths, behaving like an H1-depleted chromatin.
ISSN:1039-9712