Regulation of anthocyanin biosynthesis in strawberry (Fragaria SP.) by over-expression of a key transcription factor

Anthocyanins are secondary metabolites found in higher plants that contribute to the colours of fruits and leaves. Previous studies, in other species, have shown the regulation of anthocyanin biosynthesis is mainly at the level of transcriptional regulation of structural genes by transcription facto...

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Published inActa horticulturae no. 1048; pp. 137 - 142
Main Authors Lin-Wang, Kui, Liu, Yuhui, Espley, R. V, Karunairetnam, S, McGhie, T. K, Hellens, R. P, Allan, A. C
Format Journal Article
LanguageEnglish
Published International Society for Horticultural Science 01.01.2014
Subjects
anthocyanins
Arabidopsis thaliana
biosynthesis
color
Fragaria vesca
fruits
gene overexpression
genetically modified organisms
high performance liquid chromatography
leaves
proteins
rapid methods
secondary metabolites
strawberries
structural genes
transcription (genetics)
transcription factors
transcriptional activation
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Summary:Anthocyanins are secondary metabolites found in higher plants that contribute to the colours of fruits and leaves. Previous studies, in other species, have shown the regulation of anthocyanin biosynthesis is mainly at the level of transcriptional regulation of structural genes by transcription factors that include MYB, bHLH and WD-repeat proteins. Here we report that stable transformants of strawberry over-expressing 35S:FaMYB10 (Fragaria × ananassa) had significant increases of anthocyanins in fruits and leaves. HPLC analysis of fruits and leaves showed 70% increase of anthocyanin content in 35S:FaMYB10 transgenic lines compared with the wild-type controls. In transient transactivation assays, FaMYB10 and FvMYB10 (Fragaria vesca), co-expressed with AtbHLH2 (Arabidopsis thaliana), strongly activated the AtDFR promoter. By using the available Fragaria genome, transient expression of gene candidates, and stable transgenics, use of strawberry can provide rapid assessment of fruit gene function.
Bibliography:http://www.actahort.org/books/1048/1048_16.htm
ISSN:0567-7572