Sperm motility of masu salmon, Oncorhynchus masou, in coelomic and artificial coelomic fluids

For artificial fertilization in salmonids, the artificial coelomic fluid (ACF) consisting of 152.6 mM NaCl, 3.5 mM KCl, 2.3 mM CaCl2, 0.7 mM MgCl2, and 5 mM NaHCO3, which corresponds in ionic constituents to the coelomic fluid (CF) of salmonids, has been used to wash off yolk contaminants from eggs...

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Bibliographic Details
Published inAquaculture Science Vol. 37; no. 4; pp. 235 - 239
Main Authors Ohta, H. (Hokkaido. Fish Hatchery, Eniwa (Japan)), Matsubara, T, Imada, K
Format Journal Article
LanguageJapanese
English
Published Japanese Society for Aquaculture Science 1989
Subjects
ARTIFICIAL INSEMINATION
BODY CAVITIES
BODY FLUIDS
CAVIDADES ESPLACNICAS
CAVITE DU CORPS
CONSERVACION DEL SEMEN
CONSERVATION DU SPERME
INSEMINACION ARTIFICIAL
INSEMINATION ARTIFICIELLE
LAVADO
LAVAGE
LIQUIDE BIOLOGIQUE
LIQUIDOS CORPORALES
MOUVEMENT
MOVEMENT
MOVIMIENTO
ONCORHYNCHUS MASOU
OVA
OVULE
OVULO
SEMEN
SEMEN PRESERVATION
SOLUCION
SOLUTION
SOLUTIONS
SPERME
WASHING
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Summary:For artificial fertilization in salmonids, the artificial coelomic fluid (ACF) consisting of 152.6 mM NaCl, 3.5 mM KCl, 2.3 mM CaCl2, 0.7 mM MgCl2, and 5 mM NaHCO3, which corresponds in ionic constituents to the coelomic fluid (CF) of salmonids, has been used to wash off yolk contaminants from eggs to raise the fertilization rate. For the purpose of improving the isotonic solution, motility of spermatozoa of the masu salmon, Oncorhynchus masou, was compared between CF and ACF. The time until all spermatozoa had ceased their forward movement was longer in ACF (346.5±16.4 sec) than in CF (263.5±15.8 sec), while the percentage of motile spermatozoa was significantly higher in CF than in ACF during the time from 15 to 120 sec after dilution. The speed of movement of spermatozoa was also higher in CF than in ACF at 30 and 60 sec after dilution. The results indicate that a relatively small number of spermatozoa could continue their forward movement for a long time in ACF. The moving distance index, which was calculated from the total amount of the mean velocity × time × % motility, in ACF was about one-half that in CF. It is concluded that ACF of masu salmon lacks some element, which favours the motility of spermatozoa in CF.
Bibliography:1998000324
L53
M12
ISSN:0371-4217
2185-0194
DOI:10.11233/aquaculturesci1953.37.235