cSNP Mining from Full-length Enriched cDNA Libraries of the Korean Native Pig

• Published in: Korean Journal of Genetics Vol. 27; no. 4
• Main Authors: Dirisala, Vijaya R. (Konkuk University, Seoul, Republic of Korea), Kim, J.H. (Konkuk University, Seoul, Republic of Korea), Park, K.H. (Konkuk University, Seoul, Republic of Korea), Kim, N.E. (Konkuk University, Seoul, Republic of Korea), Lee, K.T. (National Livestock Research Institute, RDA, Suwon, Republic of Korea), Oh, S.J. (National Livestock Research Institute, RDA, Suwon, Republic of Korea), Oh, J.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Kim, N.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Um, S.J. (Konkuk University, Seoul, Republic of Korea), Lee, H.T. (Konkuk University, Seoul, Republic of Korea), Kim, K.I. (Cheju National University, Jeju, Republic of Korea), Park, C.K. (Konkuk University, Seoul, Republic of Korea), E-mail: chankyu@konkuk.ac.kr
• Format: Journal Article
• Language: English
• Published: 01.12.2005
• Subjects: CERDO; ESTs; full-length cDNA libraries; Korean native pig; PORCIN; SNPs; SWINE
• ISSN: 0254-5934

Full-length enriched cDNA libraries from spleen and brainstem tissues of a Korean native pig were constructed in an effort to characterize its genome. The frequency of translation initiation site containing clones for spleen and brainstem libraries was 60% and 80% respectively. Unreported 5' untranslated regions (UTRs) were identified from some of the selected clones, showing these libraries have full-length enriched characteristics. Chromatograms of 40,000 pig expressed sequence tags (ESTs) were retrieved from the GenBank trace file archive and combined with 1,205 EST sequences generated in this study.