Identification of Differentially Expressed Genes Involved in Spine Formation on Seeds of Daucus carota L. (Carrot), Using Annealing Control Primer (ACP) System

Carrot seeds normally have surface spines. The availability of a spineless mutant would be agronomically beneficial, eliminating the current efforts to remove those spines pre-sowing. Furthermore, the identification of spine-specific genes would provide insights into spine development in wild-type c...

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Published inJournal of plant biology = Singmul Hakhoe chi Vol. 49; no. 2
Main Authors Park, J.S. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea), Kim, I.S. (Keimyung University, Daegu, Republic of Korea), Cho, M.S. (Daegu University, Gyeongsan, Republic of Korea), Park, S. (Daegu University, Gyeongsan, Republic of Korea), Park, S.G. (Daegu University, Gyeongsan, Republic of Korea), E-mail: sgpark@daegu.ac.kr
Format Journal Article
LanguageEnglish
Published 30.04.2006
Subjects
annealing control primer (ACP)
CAROTTE
CARROTS
differentially expressed gene (DEG)
RT-PCR
spineless
spiny
ZANAHORIA
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Summary:Carrot seeds normally have surface spines. The availability of a spineless mutant would be agronomically beneficial, eliminating the current efforts to remove those spines pre-sowing. Furthermore, the identification of spine-specific genes would provide insights into spine development in wild-type carrot seed. This effort could be facilitated through the use of an annealing control primer (ACP) system. Here, we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves ACPs for identifying genes of interest.
Bibliography:2007000592
F60
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03031009