Induction and Purification of a Thermophilic Chitinase Produced by Aeromonas sp. DYU-Too7 Using Glucosamine

In the presence of chitin, Aeromonas sp. DYU-Too7 can produce extra-cellular, chitin-degrading enzymes. Chitin analogues and other carbon sources can be used to cultivate this bacterial strain. The chitinases produced by the strain were higher in the GlcN (glucosamine) medium than those in other med...

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Published inBiotechnology and bioprocess engineering Vol. 12; no. 6
Main Authors Lien, T.S. (Da-Yeh University, Changhua, Taiwan ROC), Yu, S.T. (Da-Yeh University, Changhua, Taiwan ROC), Wu, S.T. (Da-Yeh University, Changhua, Taiwan ROC), Too, J.R. (Da-Yeh University, Changhua, Taiwan ROC), E-mail: jrtoo@mail.dyu.edu.tw
Format Journal Article
LanguageEnglish
Published 01.11.2007
Subjects
aeromonas sp. DYU-Too7
AMINO SUGARS
AMINOAZUCARES
AMINOSUCRE
CHITIN
CHITINASE
CHITINE
QUITINA
QUITINASA
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Summary:In the presence of chitin, Aeromonas sp. DYU-Too7 can produce extra-cellular, chitin-degrading enzymes. Chitin analogues and other carbon sources can be used to cultivate this bacterial strain. The chitinases produced by the strain were higher in the GlcN (glucosamine) medium than those in other media. The maximal chitinase activity occurred in the medium containing 0.1% GlcN. Cultivation of Aeromonas sp. DYU-Too7 in the GlcN medium sped up the chitinase production; however, the same result did not appear when it was cultivated in the (Chitin + GlcN) medium.
Bibliography:2008001687
E21
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02931076