Detection of Salmonella enterica in food on the next day after the sample collection using 5 nuclease polymerase chain reaction with end-point fluorimetry

A method for the detection of Salmonella enterica in food on the next day after the sample collection is presented. The method consists of a two-step enrichment, bacterial cells lysis and a closed-tube duplex 5 nuclease PCR with an internal amplification control, which is read in an end-point mode i...

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Published inJournal of food and nutrition research Vol. 45; no. 1
Main Authors Krascsenicsova, K.(Food Research Institute, Bratislava (Slovak Republic), Kaclikova, E.Food Research Institute, Bratislava (Slovak Republic), Pangallo, D.Slovak Academy of Sciences, Bratislava (Slovak Republic). Institute of Molecular Biology, Siekel, P.Food Research Institute, Bratislava (Slovak Republic), Girotti, S.University of Bologna, Bologna (Italy). University of Chemical Sciences, Kuchta, T.Food Research Institute, Bratislava (Slovak Republic)
Format Journal Article
LanguageEnglish
Published 2006
Subjects
ALIMENTOS
FOODS
PCR
PRODUIT ALIMENTAIRE
REPUBLICA ESLOVACA
REPUBLIQUE SLOVAQUE
SALMONELLA ENTERITIDIS
SALMONELLA TYPHIMURIUM
SLOVAK REPUBLIC
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Summary:A method for the detection of Salmonella enterica in food on the next day after the sample collection is presented. The method consists of a two-step enrichment, bacterial cells lysis and a closed-tube duplex 5 nuclease PCR with an internal amplification control, which is read in an end-point mode in a 96-well fluorimeter. The 5-nuclease PCR has an intrinsic detection limit of 10E4 CFU/ml and facilitates monitoring of false negative results caused by the failure of amplification. The complete method for the detection of S. enterica in food has a detection limit of 100 CFU/25 g and does not produce false positive results in case of the presence of dead cells.
Bibliography:Q03
2006000161
ISSN:1336-8672