In vitro shoot proliferation competence of apple rootstocks M. 9 and M. 26 on different carbon sources

Competence of two apple rootstocks M. 9 and M. 26 for in vitro shoot proliferation was appraised using a miscellany of carbon sources i.e., sorbitol, sucrose, glucose and mannitol which were employed @ 0, 5, 15, 25, 35 and 45 g l-1. The most auspicious outcome was achieved by sorbitol @ 35 g l-1 (T9...

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Published inPakistan journal of botany Vol. 41; no. 4; pp. 1781 - 1795
Main Authors Yaseen, M. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture), Ahmed, T. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture), Abbasi, N.A. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture), Hafiz, I.A
Format Journal Article
LanguageEnglish
Published 01.08.2009
Subjects
APPLES
CULTURE MEDIA
GLUCOSE
GROWTH
IN VITRO CULTURE
MALUS
MANNITOL
MERISTEM CULTURE
ROOTSTOCKS
SORBITOL
SUCROSE
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Summary:Competence of two apple rootstocks M. 9 and M. 26 for in vitro shoot proliferation was appraised using a miscellany of carbon sources i.e., sorbitol, sucrose, glucose and mannitol which were employed @ 0, 5, 15, 25, 35 and 45 g l-1. The most auspicious outcome was achieved by sorbitol @ 35 g l-1 (T9) being the optimal carbon source for both the genotypes. M. 26 had a positive interaction with sorbitol at this concentration to produce the best caulogenic response in terms of a paramount shoot length (3.01 cm) and an overriding fresh weight increment (402 mg) whereas M. 9 at the same concentration gave an eminent shoot number (9.8). Sucrose and glucose also had a positive carryover effect on apple shoots to some extent but proved to be inferior to sorbitol. Results yielded by mannitol were highly indigent in comparison to other carbon sources. Rootstocks exhibited an inconsistency regarding their aptitude for shoot proliferation. M. 26 was recognized as a better rootstock with an acquisition of 1.05 cm shoot length and 154.6 mg fresh weight while M. 9 stood better with maximum shoot number of 2.3.
Bibliography:F02
CATPAK-78163
ISSN:0556-3321