Chromosomal homologies between Drosophila lebanonensis and D. melanogaster determined by in situ hybridization

Twelve biotin-labelled recombinant DNA probes were hybridized to polytene chromosomes of Drosophila melanogaster and D. lebanonensis. Probes were chosen in order to cover the whole chromosomal complement. Six probes correspond to known genes from D. melanogaster (RPII215, H3-H4, MHC, hsp28/23, hsp83...

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Bibliographic Details
Published inChromosoma Vol. 102; no. 5; pp. 361 - 368
Main Authors Papaceit, M, Juan, E
Format Journal Article
LanguageEnglish
Published Austria 01.05.1993
Subjects
Alcohol Dehydrogenase - genetics
Animals
Chromosome Banding
Chromosomes
cytogenetic analysis
DNA Probes
Drosophila
Drosophila - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
evolution
In Situ Hybridization
Karyotyping
nucleic acid hybridization
pericentric inversion
polytene chromosomes
Salivary Glands - cytology
Species Specificity
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Summary:Twelve biotin-labelled recombinant DNA probes were hybridized to polytene chromosomes of Drosophila melanogaster and D. lebanonensis. Probes were chosen in order to cover the whole chromosomal complement. Six probes correspond to known genes from D. melanogaster (RPII215, H3-H4, MHC, hsp28/23, hsp83, hsp70), four probes are clones isolated from a D. subobscura library (Xdh, lambda DsubS3, lambda DsubG3, lambda DsubG4) and the remaining two probes correspond to the Adh gene of D. lebanonensis and to one sequence (262), not yet characterized, from the same species. The chromosomal homologies obtained from the in situ hybridization results allow us to determine that Muller's C and D chromosomal elements are fused in the karyotype of D. lebanonensis and constitute the large metacentric chromosome. Single pericentric inversions in the E and B elements have generated the medium and small metacentric chromosomes, respectively. No great changes are detected in Muller's A element, which remains acrocentric. The changes detected in the karyotypic evolution of D. lebanonensis are frequently observed in Drosophila evolution, as deduced from chromosomal homologies of several Drosophila species. The results are also consistent with Muller's proposal that chromosomal elements have been conserved during the evolution of Drosophila.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00661280