Integration and expression of the WAP-hPC gene in three generations of transgenic rabbits

The aim of the present study was to obtain transgenic rabbits expressing human protein C in their mammary gland. A fusion construct which consisted of 4.2 kb long mouse whey acidic protein (WAP) promoter and 9.4 kb genomic human protein C (hPC) was microinjected into rabbit zygotes. Born animals wer...

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Bibliographic Details
Published inCzech Journal of Animal Science Vol. 47; no. 2
Main Authors Chrenek, P, Vasicek, D, Makarevich, A, Petrovicova, I, Bulla, J. (Vyskumny Ustav Zivocisnej Vyroby, Nitra (Slovak Republic)), Uhrin, P, Lubon, H, Binder, B.R
Format Journal Article
LanguageEnglish
Published 01.02.2002
Subjects
ANIMAL TRANSGENIQUE
ANIMALES TRANSGENICOS
ANTICOAGULANT
ANTICOAGULANTES
ANTICOAGULANTS
CONEJO (ORYCTOLAGUS)
ELISA
ENZIMAS
ENZYME
ENZYMES
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
GENES
IMMUNOBLOTTING
LAIT
LAPIN (ORYCTOLAGUS)
LECHE
MILK
PCR
RABBITS
TEST ELISA
TRANSGENIC ANIMALS
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Summary:The aim of the present study was to obtain transgenic rabbits expressing human protein C in their mammary gland. A fusion construct which consisted of 4.2 kb long mouse whey acidic protein (WAP) promoter and 9.4 kb genomic human protein C (hPC) was microinjected into rabbit zygotes. Born animals were subjected to PCR amplification to detect the integration of the injected construct into their genome. As examined during three generations, the founders transmitted transgenic allele in a Mendelian fashion. Western blot analysis demonstrated the presence of hPC in the milk of lactating transgenic females. The concentration of the recombinant hPC, as determined by ELISA, ranged between 0.24-0.56 microg/mL. This relatively low expression rate might be caused by the animal species used or might be a subject of position effects of the integration of the heterologous construct.
Bibliography:2002000504
L10
ISSN:1212-1819