Roles of the meta- and the ortho-Cleavage Pathways for the Efficient Utilization of Aromatic Hydrocarbons by Sphingomonas yanoikuyae B1

Catabolic pathways for the degradation of various aromatics by Sphingomonas yanoikuyae B1 are intertwined, joining at the level of substituted benzoates, which are further degraded via ring cleavage reactions. The mutant strain EK497, which was constructed by deleting a large DNA region containing m...

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Bibliographic Details
Published inThe journal of microbiology Vol. 38; no. 4
Main Authors Song Jeong-Min, Sung Jung-Hee Yonsei University, Seoul (Korea Republic)
Format Journal Article
LanguageEnglish
Published 01.12.2000
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
AROMATIC COMPOUNDS
COMPOSE AROMATIQUE
COMPUESTOS AROMATICOS
CULTIVOS INICIADORES
CULTURE STARTER
DEGRADACION
DEGRADATION
ENZIMAS
ENZYME
ENZYME ACTIVITY
ENZYMES
GENE
GENES
MUTACION
MUTATION
STARTER CULTURES
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Summary:Catabolic pathways for the degradation of various aromatics by Sphingomonas yanoikuyae B1 are intertwined, joining at the level of substituted benzoates, which are further degraded via ring cleavage reactions. The mutant strain EK497, which was constructed by deleting a large DNA region containing most of the genes for biphenyl, naphthalene, m-xylene, and m-toluate degradation, was unable to grow on all of the aromatics tested except for benzoate as the sole source of carbon and energy. S. yanoikuyae EK497 was found to possess only catechol ortho-ring cleavage activity due to deletion of the genes for the meta-cleavage pathway. Wild-type S. yanoikuyae B1 grown on benzoate has both catechol ortho- and meta-cleavage activity. However, m-xylene and m-toluate, which are metabolized through meth-ylbenzaate, and biphenyl, which is metabolized through benzoate, induce only the meta-cleavage pathway, suggesting the presence of a substrate-dependent induction mechanism.
Bibliography:2001000932
F30
ISSN:1225-8873
1976-3794