genome of the Mediterranean fruitfly Ceratitis capitata: localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes

• Published in: Chromosoma Vol. 101; no. 7; pp. 448 - 455
• Main Authors: Zacharopoulou, A, Frisardi, M, Savakis, C, Robinson, A.S, Tolias, P, Konsolaki, M, Komitopoulou, K, Kafatos, F.C
• Format: Journal Article
• Language: English
• Published: Austria 01.04.1992
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; autosomes; Base Sequence; Ceratitis capitata; Chromosome Mapping; clones; cytogenetics; Diptera - genetics; DNA; Drosophila melanogaster - genetics; embl/y00221; genbank/y00221; Genetic Markers; Genome; larvae; markers; Molecular Sequence Data; Nucleic Acid Hybridization; nucleotide sequences; polytene chromosomes; salivary glands; Salivary Glands - metabolism; X Chromosome
• ISSN: 0009-5915; 1432-0886
• DOI: 10.1007/BF00582839

We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly, Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent the X chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L of C. capitata is homologous to the Drosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.