Comparative studies on three isolates of Breda virus of calves

• Published in: American journal of veterinary research Vol. 46; no. 5; p. 1003
• Main Authors: Woode, G.N, Saif, L.J, Quesada, M, Winand, N.J, Pohlenz, J.F, Gourley, N.K
• Format: Journal Article
• Language: English
• Published: United States 01.05.1985
• Subjects: Animals; Antigen-Antibody Complex; CALVES; Cattle; Cattle Diseases - microbiology; Diarrhea - microbiology; Diarrhea - veterinary; Enzyme-Linked Immunosorbent Assay; Epitopes; Feces - microbiology; Fluorescent Antibody Technique; Hemagglutination Inhibition Tests - veterinary; Hemagglutination Tests - veterinary; IOWA; Microscopy, Electron; OHIO; Rotavirus - immunology; Rotavirus - isolation & purification; TERNERO; VEAU; VIROSE; VIROSES; VIROSIS; VIRUS; VIRUSES; Viruses, Unclassified - immunology; Viruses, Unclassified - isolation & purification; Viruses, Unclassified - ultrastructure; Iowa; Ohio
• ISSN: 0002-9645; 1943-5681

Three isolates of Breda virus of calves were compared morphologically and antigenically. The isolates demonstrated similar morphology and shared common antigens, as determined by enzyme-linked immunosorbent assay and immunoelectron microscopy. On the basis of results of the hemagglutination-inhibition test, enzyme-linked immunosorbent assay, and immunoelectron microscopy, the 3 isolates were further subdivided into 2 serotypes: serotype 1 (Breda virus 1) represented by the Iowa isolate 1; and serotype 2 (Breda virus 2), by the Ohio isolate and the Iowa isolate 2. The 3 isolates caused diarrhea in gnotobiotic calves.