Biosynthesis, production site, and emission rates of aggregation-attachment pheromone in males of two Amblyomma ticks

• Published in: Journal of chemical ecology Vol. 17
• Main Authors: Diehl P.A, Guerin P, Vlimant M, Steullet P
• Format: Journal Article
• Language: English
• Published: 01.01.1991
• Subjects: amblyomma variegatum; biosintesis; biosynthese; biosynthesis; feromonas; pheromone; pheromones
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/BF01395594

The aggregation-attachment pheromone components o-nitrophenol (ONP) and methyl salicylate (MS) in male Amblyomma variegatum ticks appeared after three days of feeding on the host and reached high values after about six days. Variable quantities of 1.3-7.3 microgram ONP and about 0.6 microgram MS were present within ticks. ONP arid MS were pleased at the high rates of 300-1800 ng/hr and 20-600 ng/hr per male tick, respectively. After a temporary decrease, males continued to emit at high rates after nearby attachment of females. In A. hebraeum, ONP showed a similar pattern, but with a delay of about a day. A male, which had fed during 14 days, contained about 2 microgram and released 225-280 ng/hr. Emission in forcibly detached males of both species dropped rapidly to low levels of less than 10 ng/hr per tick. Host skin and tick feces in the vicinity of feeding males were pheromone-impregnated. The very high emission rates are consistent with the observations that the pheromone is an important component of the host-location mechanism of conspecifics. ONP and MS ate produced in the dermal glands type 2 associated with the ventrolateral cuticle.