Comparative effect of IBA, BSAA and 5,6-Cl2-IAA-Me on the rooting of hypocotyl in mung bean

• Published in: Plant growth regulation Vol. 27; no. 2; pp. 91 - 98
• Main Authors: Pan, R, Tian, X
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1999; Springer Nature B.V
• Subjects: 3-(benzo[b]selenienyl) acetic acid; 5,6-dichloroindole-3-acetic acid; acetic acid; adventitious roots; Biological and medical sciences; chemical constituents of plants; derivatives; dose response; enzyme activity; Fundamental and applied biological sciences. Psychology; hypocotyls; IAA oxidase; indole acetic acid; indole butyric acid; Metabolism; Metabolism. Physicochemical requirements; methyl ester; paclobutrazol; peroxidase; plant cuttings; Plant physiology and development; regulation; rooting; seedlings; Vigna radiata; Populus tremuloides; Oxidative stress; Enzyme; Induction; Tissue specificity; Ozone; Superoxide dismutase; Gene expression; Salicaceae; Complementary DNA; Dicotyledones; Angiospermae; Isolation; Multigene family; Hardwood forest tree; Spermatophyta; Oxidoreductases; Sequencing
• ISSN: 0167-6903; 1573-5087
• DOI: 10.1023/A:1006154426941

Mung bean hypocotyl cuttings were treated with indole-3-butyric acid (IBA), 3-(benzo[b]selenienyl)acetic acid (BSAA) and 5,6-dichloroindole-3-acetic acid methyl ester (5,6-Cl2-IAA-Me) at different concentrations, respectively. Each chemical produced the maximum number of adventitious roots at a different concentration. Compared with IBA treatment, 5,6-Cl2-IAA-Me and BSAA treatments significantly increased root numbers on hypocotyl cuttings at lower concentration, particularly of 5,6-Cl2-IAA-Me treatment. Combinations of paclobutrazol (PB) with either 5,6-Cl2-IAA-Me or BSAA significantly stimulated the production of more adventitious roots than either chemical alone or combined. Capillary electrophoresis analysis have shown that the levels of IAA, IBA and BSAA in IBA plus PB or BSAA plus PB treatments were higher than those of IBA or BSAA alone. It was suggested that the cause of the synergistic effect of IBA (or BSAA) plus PB treatment might be due to increased endogenous auxin level. The activities of peroxidase and IAA oxidase in the rooting zone coincided with root development, indicating that the activities of these two enzymes were positively correlated to rooting. Peroxidase and IAA oxidase activity in all treatments started 24 h and 12 h after cutting, respectively. It is suggested that the major role of IAA oxidase differed from that of peroxidase in adventitious root formation.