Cryopreservation of Pacific oyster, Crassostrea gigas, sperm

Several factors which affect the post survival of cryopreserved Pacific oyster, Crassostrea gigas sperm were examined by comparing percentage of fertilized eggs after insemination of fresh and cryopreserved sperm. Highest post survival was achieved by using DMSO (dimethyl sulfoxide), as a cryo-prote...

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Bibliographic Details
Published inAquaculture Science Vol. 37; no. 3; pp. 163 - 166
Main Authors Iwata, N. (Central Research Inst. of Electric Power Industry, Tokyo (Japan)), Kurokura, H, Hirano, R
Format Journal Article
LanguageEnglish
Japanese
Published Japanese Society for Aquaculture Science 31.08.1989
Subjects
CONGELACION
CONGELATION
CONSERVACION DEL SEMEN
CONSERVATION DU SPERME
CRASSOSTREA GIGAS
FERTILIDAD
FERTILITE
FERTILITY
FREEZING
NEIGE CARBONIQUE
NIEVE CARBONICA
SEMEN PRESERVATION
SOLID CARBON DIOXIDE
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Summary:Several factors which affect the post survival of cryopreserved Pacific oyster, Crassostrea gigas sperm were examined by comparing percentage of fertilized eggs after insemination of fresh and cryopreserved sperm. Highest post survival was achieved by using DMSO (dimethyl sulfoxide), as a cryo-protectant, in 2/3 artificial sea water with 3 min. of equilibration period. The estimated fertilizing capacity of cryopreserved sperm corresponded to 1/100-1/1000 of the capacity of fresh sperm. However, this low fertilizing capacity could be compensated by increasing the number of the inseminated sperm.
Bibliography:9202248
L10
ISSN:0371-4217
2185-0194
DOI:10.11233/aquaculturesci1953.37.163