Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

• Published in: Journal of Integrative Agriculture Vol. 11; no. 11; pp. 1845 - 1851
• Main Author: LI Yi-ping, WUJun-xiang , LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan (1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China 2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China 3.Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, P.R.China 4.Ministry of Agriculture, Beijing 100026, P.R.China)
• Format: Journal Article
• Language: English
• Published: Science Press 01.11.2012; Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education/College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, P R.China%State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education/College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, P R.China; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China%Key Laboratory of Applied Entomology; Key Laboratory of Applied Entomology
• Subjects: Helicoverpa armigera Bt resistance oligomerization pore formation; Bt resistance; Helicoverpa armigera; oligomerization; pore formation
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(12)60189-0

With the long-term use of Bacillus thuringiensis (Bt) insecticide and expansion of Cry1A-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N (APN), in brush border membrane vesicles (BBMV) of Helicoverpa armigera were examined in both Cry1Ac-susceptible (96S) and Cry1Acresistant (LF120) strains, to compare Cry1Ac toxin oligomerization and pore formation in these two strains. Cry1Ac toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant H. armigera strains. The ability to form Cry1Ac oligomers and ion channels on BBMVs was also not significantly different between these two strains.