Cell wall metabolism in ripening fruit. IX. Synthesis of pectic and hemicellulosic cell wall polymers in the outer pericarp of mature green tomatoes (cv XMT-22)

• Published in: Plant physiology (Bethesda) Vol. 114; no. 4; pp. 1523 - 1531
• Main Authors: Huysamer, M, Greve, L.C, Labavitch, J.M
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.08.1997
• Subjects: Agronomy. Soil science and plant productions; AZUCARES ACIDOS; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Cell wall components; CELL WALLS; Development and Growth Regulation; DEVELOPMENTAL STAGES; Economic plant physiology; ETAPAS DE DESARROLLO; Fructification and ripening; Fructification, ripening. Postharvest physiology; Fundamental and applied biological sciences. Psychology; GALACTURONIC ACID; GLUCURONIC ACID; Growth and development; HEMICELLULOSE; HEMICELULOSA; ISOTOPE RADIOACTIF; LYCOPERSICON ESCULENTUM; MADURAMIENTO; METABOLISM; METABOLISME; METABOLISMO; MURISSAGE; PARED CELULAR; PAROI CELLULAIRE; PECTINAS; PECTINE; PECTINS; PERICARP; PERICARPE; PERICARPIO; Plant physiology and development; Plant tissues; Plants; POLIMEROS; POLYMERE; POLYMERS; Polysaccharides; PRECURSORS; RADIOACTIVE TRACERS; RADIOISOTOPES; RADIOISOTOPOS; RIPENING; STADE DE DEVELOPPEMENT; SUCRE ACIDE; SUGAR ACIDS; Sugar alcohols; Sugars; Vegetative and sexual reproduction, floral biology, fructification; Hemicellulose; Fruit; Isotope labelling; Biosynthesis; Pectin; Cell wall; Ripening; Vegetable crop; Dicotyledones; Angiospermae; Plant tissue disc; Lycopersicon esculentum; Spermatophyta; Solanaceae; Polysaccharide
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.114.4.1523

Discs of outer pericarp were excised from mature green tomato (Lycopersicon esculentum Mill.) fruit and kept in sterile tissue culture plates for 4 d, including 2 d of incubation with D-[U-13C]glucose. Cell walls were prepared and the water-soluble, pectic, and hemicellulosic polymers were extracted. Cell wall synthetic capacity was determined by gas chromatography-mass spectrometry analysis of incorporation of the heavy isotope label. The "outer" 2-mm pericarp region, which included the cuticle, had a lower cell wall synthetic capacity than the "inner" 2-mm region immediately below it (closer to the locules), based on the percentage of labeling of the neutral sugars. There were no significant differences in relative abundance of glycosidic linkages in the two tissue regions. Label was incorporated into neutral sugars and linkages typical for each polysaccharide class were identified in the cell wall preparations. Galacturonic acid and glucuronic acid were labeled to an extent similar to that of the neutral sugars in each tissue region