Carbon and energy balances of glucose fermentation with hydrogen-producing bacterium Citrobacter amalonaticus Y19

For the newly isolated H₂-producing chemoheterotrophic bacterium Citrobacter amalonaticus Y19, anaerobic glucose metabolism was studied in batch cultivation at varying initial glucose concentrations (3.5-9.5 g/l). The carbon-mass and energy balances were determined and utilized to analyze the carbon...

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Published inJournal of microbiology and biotechnology Vol. 18; no. 3; pp. 532 - 538
Main Authors Oh, Y.K. (Korea Institute of Energy Research, Daejeon, Republic of Korea), Park, S.H. (Pusan National University, Busan, Republic of Korea), E-mail: parksh@pusan.ac.kr, Seol, E.H. (Pusan National University, Busan, Republic of Korea), Kim, S.H. (Pusan National University, Busan, Republic of Korea), Kim, M.S. (Korea Institute of Energy Research, Daejeon, Republic of Korea), Hwang, J.W. (Environmental Management Corp., Incheon, Republic of Korea), Ryu, Dewey D.Y. (University of California, Davis, California, U.S.A.)
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Applied Microbiology 01.03.2008
Subjects
anaerobic glucose fermentation
BALANCE DE ENERGIA
BILAN ENERGETIQUE
Biological and medical sciences
Biotechnology
carbon and energy balances
carbon-flux analysis
Citrobacter amalonaticus
ENERGY BALANCE
Fundamental and applied biological sciences. Psychology
hydrogen production
Hydrogen
Glucose
Carbon
Carbon balance
carbon-flux analysis
carbon and energy balances
Energy balance
anaerobic glucose fermentation
Citrobacter amalonaticus
Production
Bacteria
Anaerobic fermentation
Citrobacter
Hydrogen production
Enterobacteriaceae
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Summary:For the newly isolated H₂-producing chemoheterotrophic bacterium Citrobacter amalonaticus Y19, anaerobic glucose metabolism was studied in batch cultivation at varying initial glucose concentrations (3.5-9.5 g/l). The carbon-mass and energy balances were determined and utilized to analyze the carbon metabolic-pathways network. The analyses revealed (a) variable production of major metabolites (H₂, ethanol, acetate, lactate, CO₂, and cell mass) depending on initial glucose levels; (b) influence of NADH regeneration on the production of acetate, lactate, and ethanol; and (c) influence of the molar production of ATP on the production of biomass. The results reported in this paper suggest how the carbon metabolic pathway(s) should be designed for optimal H₂ production, especially at high glucose concentrations, such as by blocking the carbon flux via lactate dehydrogenase from the pyruvate node.
Bibliography:A50
2009000080
ISSN:1017-7825