Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd

• Published in: Bioorganic & medicinal chemistry letters Vol. 22; no. 12; pp. 4014 - 4017
• Main Authors: Li, Yan, Li, Lianzhi, Pu, Xuewei, Ma, Guolin, Wang, Erqiong, Kong, Jinming, Liu, Zhipeng, Liu, Yangzhong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 15.06.2012; Elsevier
• Subjects: binding capacity; Biological and medical sciences; cadmium; chelation; detection limit; energy transfer; fluorescence; Medical sciences; metal ions; Pharmacology. Drug treatments; stoichiometry; Cd; Dansyl; Ratiometric fluorescent sensor; Fluorescence detector; Peptides; peptidyl chemosensor; Selectivity; Chemical sensor; Fluorescence spectrometry; Resonant energy transfer; Cadmium II Compounds; FRET; Detection; Chemical synthesis; Metal binding peptide
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2012.04.088

A novel ratiometric fluorescent peptidyl chemosensor (Dansyl-Cys-Pro-Gly-Cys-Trp-NH₂, D-P5) for metal ions detection has been synthesized via Fmoc solid-phase peptide synthesis. The chemosensor exhibited a high selectivity for Cd²⁺ over other metal ions including competitive transition and Group I and II metal ions in neutral pH. The fluorescence emission intensity of D-P5 was significantly enhanced in the presence of Cd²⁺ by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The binding stoichiometry, detection limit, binding affinity, reversibility and pH sensitivity of the sensor for Cd²⁺ were investigated.