Cloning and Characterization of a Gene Cluster for Cyclohexanone Oxidation in Rhodococcus sp. TK6

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 4; pp. 511 - 518
• Main Authors: Choi, J.H. (Kyungpook National University, Daegu, Republic of Korea), Kim, T.K. (Kyungpook National University, Daegu, Republic of Korea), Kim, Y.M. (Kyungpook National University, Daegu, Republic of Korea), Kim, W.C. (Kyungpook National University, Daegu, Republic of Korea), Park, K.B. (Kyungpook National University, Daegu, Republic of Korea), Rhee, I.K. (Kyungpook National University, Daegu, Republic of Korea), E-mail: ikrhee@knu.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.04.2006
• Subjects: Biological and medical sciences; Biotechnology; cyclohexanone monooxygenase; Cyclohexanone oxidation; Fundamental and applied biological sciences. Psychology; RHODOCOCCUS (BACTERIA); Rhodococcus sp. TK6; Characterization; Gene cluster; Cyclohexanone oxidation; Rhodococcus sp. TK6; Enzyme; Cyclohexanone monooxygenase; Bacteria; Rhodococcus; Actinomycetes; Oxidation; Oxidoreductases
• ISSN: 1017-7825

A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), ε-caprolactone hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE).