Characterization of nitroreductase purified from TNT-degrading bacterium, Pseudomonas sp. HK-6

In this study nitroreductase from Pseudomonas sp. HK-6 capable of degrading 2,4,6-trinitrotoluene (TNT) was characterized. Through a series of purification process including ammonium sulfate precipitation, DEAE-sepharose, and Q-sepharose, three different fractions I, Ѧ, and Ѧ having the enzyme act...

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Bibliographic Details
Published inKorean Journal of Microbiology and Biotechnology Vol. 32; no. 3
Main Authors Ho, E.M, Kang, H.Y, Oh, K.H. (Soonchunhyang University, Sunchon, Republic of Korea)
Format Journal Article
LanguageKorean
Published 01.09.2004
Subjects
PSEUDOMONAS
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Summary:In this study nitroreductase from Pseudomonas sp. HK-6 capable of degrading 2,4,6-trinitrotoluene (TNT) was characterized. Through a series of purification process including ammonium sulfate precipitation, DEAE-sepharose, and Q-sepharose, three different fractions I, Ѧ, and Ѧ having the enzyme activity of NTRs whose molecular weights were approximately 27 kDa were detected in fractions from HK-6 cells.
Bibliography:2004008744
L10
ISSN:1598-642X