Occurrence of GalNAcbeta1-4GlcNAc unit in N-glycan of royal jelly glycoprotein

• Published in: Bioscience, biotechnology, and biochemistry Vol. 66; no. 9; pp. 1985 - 1989
• Main Authors: "Kimura, M. (Okayama Univ. (Japan). Faculty of Agriculture), Hama, Y, Tsumura, K, Okihara, K, Sugimoto, H, Yamada, H, Kimura, Y.
• Format: Journal Article
• Language: English
• Published: England 01.09.2002
• Subjects: ANIMAL GLANDS; Animals; APIS MELLIFERA; Bees - chemistry; Bees - enzymology; Carbohydrate Sequence; CHEMICAL STRUCTURE; Chromatography, High Pressure Liquid; Fatty Acids - chemistry; GLYCOPROTEINS; Glycoproteins - chemistry; Insect Hormones - chemistry; Insect Proteins - chemistry; Magnetic Resonance Spectroscopy; Molecular Sequence Data; NMR SPECTROSCOPY; Polysaccharides - analysis; Polysaccharides - chemistry; ROYAL JELLY; TRANSFERASES
• ISSN: 0916-8451; 1347-6947

"Elsewhere, we characterized the structure of twelve N-glycans purified from royal jelly glycoproteins (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000)). Structural analysis showed that the typical high-mannose type structure (Man9-4GlcNAc2) accounts for about 72% of total N-glycans, a biantennary-type structure (GlcNAc2Man3GlcNAc2) about 8%, and a hybrid-type structure (GlcNAc1Man4GlcNAc2) about 3%. During structural analysis of minor N-glycans of royal jelly glycoproteins, we found that one had an N-acetyl-galactosaminyl residue at the non reducing end; most of such residues have been found in N-glycans of mammalian glycoproteins. By exoglycosidase digestion, methylation analysis, ion-spray (IS)-MS analysis, and 1H NMR spectroscopy, we identified the structure of the N-glycan containing GaINAc as; GIcNAcbeta1-2Manalpha1-6(Gal-NAcbeta1-4GIcNAcbeta1-2Manalpha1-3)Manbeta1-4GIcNACbeta1-4GIcNAc. This result suggested that a beta1-4GaINAc transferase is present in hypopharyngeal and mandibular glands of honeybees. "