Analysis of the Cytotoxicity of Four Dentin Bonding Agents on Gingival Fibroblasts

The objective of this study is to evaluate effects of four dentin bonding agents PromptL-Pop, Syntac, Pekabond, Scotchbond-1 on cell proliferation in human gingival fibroblast cultures. Under aseptic conditions, test specimens were placed in the centre of 24-well tissue culture trays. Each well was...

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Bibliographic Details
Published inTurkish journal of medical sciences Vol. 35; no. 6; pp. 395 - 399
Main Authors ÖZEN, Jülide, ATAY, Arzu, TOPÇU, Fulya TOKSOY, URAL, Ali Uğur
Format Journal Article
LanguageEnglish
Published Turkish Journal of Medical Sciences 2005
TÜBİTAK
Subjects
Cell Culture Techniques
Dentin bağlayıcı ajanlar
Dentin bonding agents, gingival fibroblasts, MTT, cell culture
Dentin-Bonding Agents
Dentistry
Diş hekimliği
Dişeti
Fibroblastlar
Fibroblasts
Gingiva
Hücre kültür teknikleri
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Summary:The objective of this study is to evaluate effects of four dentin bonding agents PromptL-Pop, Syntac, Pekabond, Scotchbond-1 on cell proliferation in human gingival fibroblast cultures. Under aseptic conditions, test specimens were placed in the centre of 24-well tissue culture trays. Each well was covered at a concentration of 104 cells/cm2. The cultures were incubated at 37 ºC and cell proliferation was determined by the MTT method 24 h and 72 h after exposure. Statistical analysis was performed applying the Student t test. Statistical analysis of data showed that all materials caused noncytotoxic effects for 24 hours and cytoxic effects for 72 hours. Prompt L-pop displayed the highest number of cells whereas the lowest number of cells was found for Pekabond for 72 hours. These results support the proposal that some bonding agents may cause cytotoxic reactions under in vitro conditions.
Bibliography:http://dergipark.ulakbim.gov.tr/tbtkmedical/article/view/5000031006
TTIP
ISSN:1303-6165
1300-0144