Crystal structure at 1.7 angstrom of the bovine papillomavirus-1 E2 DNA-binding domain bound to its DNA target

The dominant transcriptional regulator of the papillomaviruses, E2, binds to its specific DNA target through a previously unobserved dimeric antiparallel beta-barrel. The DNA is severely but smoothly bent over the barrel by the interaction of successive major grooves with a pair of symmetrically dis...

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Bibliographic Details
Published inNature (London) Vol. 359; no. 6395; pp. 505 - 512
Main Authors Hegde, R.S, Grossman, S.R, Laimins, L.A, Sigler, P.B
Format Journal Article
LanguageEnglish
Published 08.10.1992
Subjects
Bovine papillomavirus
DNA
molecular sequence data
nucleotide sequences
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Summary:The dominant transcriptional regulator of the papillomaviruses, E2, binds to its specific DNA target through a previously unobserved dimeric antiparallel beta-barrel. The DNA is severely but smoothly bent over the barrel by the interaction of successive major grooves with a pair of symmetrically disposed alpha-helices. The specific interface is an "interwoven" network of interactions where the identifying base pairs of the target contact more than one amino-acid side chain and the discriminating amino acids interact with more than one base pair.
ISSN:0028-0836
1476-4687
DOI:10.1038/359505a0