Molecular genetics of growth and development in Populus. 3. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers

Three DNA-based marker types were evaluated for linkage map construction in Populus: RFLPs detected by Southern blot hybridization, STSs detected by a combination of PCR and RFLP analysis, and RAPDs. The mapping pedigree consists of three generations, with the F1 produced by interspecific hybridizat...

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Published inTheoretical and applied genetics Vol. 89; no. 2-3
Main Authors Bradshaw, H.D. Jr. (Washington Univ., Seattle (USA). Dept. of Biochemistry), Villar, M, Watson, B.D, Otto, K.G, Stewart, S, Stettler, R.F
Format Journal Article
LanguageEnglish
Published 01.10.1994
Subjects
Aigeiros Salix
CARTE GENETIQUE
Cottonwood
CRECIMIENTO
CROISSANCE
GENETICA MOLECULAR
GENETIQUE MOLECULAIRE
GENOMAS
GENOME
MAPAS GENETICOS
PCR
POPULUS DELTOIDES
POPULUS TRICHOCARPA
RAPD
RFLP
Tacamahaca
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Summary:Three DNA-based marker types were evaluated for linkage map construction in Populus: RFLPs detected by Southern blot hybridization, STSs detected by a combination of PCR and RFLP analysis, and RAPDs. The mapping pedigree consists of three generations, with the F1 produced by interspecific hybridization between a Populus trichocarpa female and a Populus deltoides male. The F2 generation was made by inbreeding to the maximum degree permitted by the dioecious mating system of Populus. The applicability of STSs and RAPDs outside the mapping pedigree has been investigated, showing that these PCR-based marker systems are well-suited to breeding designs involving interspecific hybridization. A Populus genome map (343 markers) has been constructed from a combination of all three types. The length of the Populus genome is estimated to be 2400-2800cM.
Bibliography:F30
K10
94B1573
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00225137