Quantification of Bacillus thuringiensis insect control protein as expressed in transgenic plants

Quantitative assays were developed to analyze gene products produced by transgenic plants that express Bacillus thuringiensis insect control proteins. Initially B. thuringiensis proteins were produced in plants at approximately 10,000 fold lower specific activities than the same proteins produced by...

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Bibliographic Details
Published inACS symposium series no. 432
Main Authors Fuchs, R.L. (Monsanto Agricultural Company, St. Louis, MO), MacIntosh, S.C, Dean, D.A, Greenplate, J.T, Perlak, F.J, Pershing, J.C, Marrone, P.G, Fischhoof, D.A
Format Journal Article
LanguageEnglish
Published 1990
Subjects
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
BACILLUS THURINGIENSIS
CONTROL BIOLOGICO
CONTROL DE INSECTOS
ENDOTOXINAS
ENDOTOXINE
EXPRESION GENICA
EXPRESSION DES GENES
GENIE GENETIQUE
INGENIERIA GENETICA
LUTTE ANTIINSECTE
LUTTE BIOLOGIQUE
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
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Summary:Quantitative assays were developed to analyze gene products produced by transgenic plants that express Bacillus thuringiensis insect control proteins. Initially B. thuringiensis proteins were produced in plants at approximately 10,000 fold lower specific activities than the same proteins produced by microbial fermentations. Consequently, accurate and selective assays were developed to quantitate B. thuringiensis specific DNA (gene copy), mRNA and protein produced by insect tolerant transgenic plants. The 100 fold increase in B. thuringiensis protein expression achieved in plants, based on these analysis, enabled the production of transgenic plants that are protected against agronomically important insect pests
Bibliography:H01
9305257
F30
ISSN:0097-6156
1947-5918