해조류 구멍갈파래 (Ulva pertusa )의 친환경적 이용을 위한 바이오에탄올의 생산

• Published in: KSBB Journal Vol. 26; no. 4; pp. 317 - 322
• Main Authors: 김종덕, 윤양호, 신태선, 김민용, 변헌수, 오석진, 서효진
• Format: Journal Article
• Language: Korean
• Published: 한국생물공학회 2011
• Subjects: H2SO4; Combined strain; Seashore waste; Ethanol; P. stipitis; Rhamnose; Ulva pertusa; S. cerevisiae; H₂SO; P; S; H_2SO_4; cerevisiae; stipitis
• ISSN: 1225-7117; 2288-8268

Ulva pertusa is one of the worst pollutant like a waste vinyl after agriculture and caused bad smell at seashore in Jejudo and south area of korean peninsular. For favorable environmental utilization of Ulva pertusa, it could be applied for ethanol production with its acid hydrolysate. The components of hydrolysate included fermentable sugar of glucose, xylose, mannose, galactose, and higher amounts of unfermentable rhamnose. Fermentable sugars were converted to ethanol with S. cerevisiae, also xylose to ethanol with P. stipitis, their maximun ethanol production at optimum conditions were 462 μg/mL and 475 μg/mL, respectively. While, rhamnose cannot be changed to ethanol with S. cerevisiae or P. stipitis, alone. Combination of S. cerevisiae and P. stipitis can convert rhamnose to ethanol, because P.stipitis degradaded rhamnose to pyruvate, and then S. cerevisiae convert to ethanol, at optimum conditions, ethanol reached to 782 μg/mL (30.24%) that is higher than that of 2 strain alone from 500 mg of dried Ulva pertusa contained 2586.45 μg/mL of reduced sugars. Ulva pertusa can be utilized for renewal energy insted of environmenatal enemy.